The substances were precipitated using saturated ammonium sulfate solution, redissolved in Tris-HCl buffer, and then filtered using a 100 kDa ultrafiltration cube, the results showed that the active components of the substance was trapped by the ultrafitration cube. 该活性物质经硫酸铵盐析沉淀,沉淀复溶于Tris-HCl缓冲液后,使用100KDa超滤管进行超滤,该物质被截留,说明其分子量大于100KDa。